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Plant tissue culture is a set of techniques that can be applied in various fields, including basic research, germplasm conservation, and commercial applications such as micropropagation, crop improvement, and secondary metabolite production. This short course on plant tissue culture provides hands-on training and an understanding on laboratory practices and transfers from the lab to the field. This course is suitable for school teachers, technical college instructors, entrepreneurs looking to establish their labs, and researchers in plant tissue culture focusing on commercial applications. The plant tissue culture sector has great potential to become a widespread income-generating business, providing more job opportunities to the community.